Bro: you probably have lost the job. 1, it shows that you are not serious or professional enough 'coz all you think is "asking for leave" rather than "how to get work started". 2, it is a common sense that new employees won't have any personal vacation in the first 6 months. If you don't know this, they would doubt your prior work experience.
Back up plan: Call them or email them to tell them how much this job meant to you.
Bro: however, I admire your courage. People put different things in the priority list. You put family in the fist priority, which set a good model to young generations. However, this world has changed. Be smart, so that you could survive.