Because she filed I130 to sponsor her brother immigration visa application, her brother has the immigration intent and thus, if he applys for b1/b2 visa, the visa officer (VO) will deny his b1/b2 visa application, but this b1/b2 visa applicaiton denial will not affect his future immigration visa application. Of course, if the VO is drunk and thus issue the b1/b2 visa to his brother, he can use the B1/B2 visa to enter US without affecting his future immigration visa application.
But his broher's wife and child can apply for the b1/b2 visa at this stage.
Is that clear?