There is not a rule says about 180days. It is just based on the common sense.
(前后两份工作都是non-profit组织)在以后的485审批中会不会的麻烦?
It is different way.
新H1申请一递交上去,在等待审批期间是不是不算是out of status?
You are in the AOS, so it is not out of status. If you worried about it, just use EAD.
2 如果用EAD工作,以后是否会被因为在递交485不到180天就用EAD工作影响绿卡审批?
The answer is on the above.