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Biopsy under CT guidance is performed by interventional radiologist, not pulmonologist, neither thoracic oncologist

In your case, an isolated lung nodule could be all kinds of possibility.

1 benign. Could be benign tumor or infection

2 malignancy. Could be lung originated cancer, or other solid cancer metastasis to lung, or hematologic cancer such as lymphoma

The treatment for the above possibilities are different

Thus I think biopsy is a must and thinking to much details of the procedure of biopsy will exhaust you and really not nessary in my opion

I wish you the best